| Airline | Country | Status |
| AB Aerotransport | Sweden | D |
| Ad Astra Aero | Switzerland | D |
| Aero Espresso Italiana | Italy | D |
| Aero Geral | Brazil | D |
| Aero O/Y | Finland | F |
| Aeroflot | Soviet Union | F |
| Aerolíneas Argentinas | Argentina | F |
| Aeromarine Airways | United States | D |
| Aeromarine West Indies Airways | United States | D |
| Aéropostale | France | D |
| Air Fecteau | Canada | D |
| Air France | France | F |
| Air Juan | Philippines | C |
| Air Labrador | Canada | D |
| Air Mikisew | Canada | D |
| Air Orient | France | D |
| Air Saguenay | Canada | D |
| Air Tahiti | France | F |
| Air Tindi | Canada | C |
| Air Union | France | D |
| Air Whitsunday | Australia | C |
| AirSea Lines | Greece | D |
| Ala Littoria | Italy | D |
| Alaska Airlines | United States | F |
| Alaska Seaplanes[2] | United States | C |
| Alkan Air | Canada | C |
| Ansett Australia | Australia | D |
| Antilles Air Boats | United States | D |
| Aquila Airways | United Kingdom | D |
| Arctic Sunwest Charters | Canada | D |
| Austin Airways | Canada | D |
| Baxter Aviation | Canada | D
|
| British Amphibious Airlines | United Kingdom | D |
| British Aviation Services | United Kingdom | D |
| British Marine Air Navigation Co | United Kingdom | D |
| British Overseas Airways Corporation | United Kingdom | D |
| Buffalo Airways | Canada | F |
| Canadian Airways | Canada | D |
| Catalina Air Lines | United States | D |
| Cathay Pacific | Hong Kong | F |
| Chalk's International Airlines | United States | D |
| China National Aviation Corporation (CNAC) | China | D |
| Comité d' Etude pour la Navigation Aérienne du Congo (CENAC) | Belgium/Belgian Congo | D |
| Conair Group | Canada | C |
| Condor Syndikat | Germany | D |
| Corilair | Canada | C |
| Curtiss Flying Service | United States | D |
| Deruluft | Germany/Soviet Union | D |
| Deutsche Luft Hansa | Germany | D |
| Dobrolyot | Soviet Union | D |
| Eastern Provincial Airways | Canada | D |
| Elders Colonial Airways | Nigeria | D |
| Eldorado Radium Silver Express | Canada | D |
| European Coastal Airlines | Croatia | D |
| Fjellfly | Norway | D |
| Fly Lapland Oy[3] | Finland | C |
| Garuda Indonesia | Indonesia | F |
| Golden West Airlines | United States | D |
| Harbour Air Seaplanes | Canada | C |
| Hellenic Seaplanes | Greece | C |
| Imperial Airways | United Kingdom | D |
| Imperial Japanese Airways | Japan | D |
| Inter-Island Airways | United States | F |
| Jal Hans | India | C |
| Japan Air Transport | Japan | C |
| Jersey Airways | Channel Islands | D |
| Kenmore Air | United States | C |
| Kenn Borek Air | Canada | C |
| Kerala Seaplane | India | C |
| KLM | Netherlands | F |
| KLM Interinsulair Bedrijf | Dutch East Indies | D |
| KNILM | Netherlands | D |
| Lamb Air | Canada | D |
| LAN Airlines | Chile | F |
| Laurentide Air Services | Canada | D |
| Lignes Aériennes Latécoère | France | D |
| Loch Lomond Seaplanes | United Kingdom | C |
| Macau Air Transport Company | Hong Kong | D |
| Maldivian | Maldives | C |
| Maldivian Air Taxi | Maldives | D |
| Mørefly | Norway | D |
| Mount Cook Airline | New Zealand | F |
| Nakina Air Service | Canada | C |
| New York, Rio, and Buenos Aires Line (NYRBA) | United States | D |
| Norcanair | Canada | D |
| Nordair | Canada | D |
| Nordic Seaplanes[4] | Denmark | C |
| North-Wright Airways | Canada | C |
| Northwest Airlines | United States | D |
| Northwestern Air | Canada | C |
| Norwegian Air Lines | Norway | D |
| Ontario Central Airlines | Canada | D |
| Ontario Provincial Air Service | Canada | D |
| Pacific Island Air | Fiji | C |
| Pacific Seaplanes[5] | Canada | C |
| Pacific Western Airlines | Canada | D |
| Pan American World Airways | United States | D |
| Panair do Brasil | Brazil | D |
| Paraense Transportes Aéreos | Brazil | D |
| Pat Bay Air | Canada | D |
| Pearl Aviation | Australia | F |
| PenAir | United States | F |
| Qantas | Australia | F |
| Quebecair | Canada | D |
| Queen Charlotte Airlines | Canada | D |
| Salt Air | New Zealand | C |
| Salt Spring Air | Canada | C |
| Samaritan Aviation | Papua New Guinea | C |
| SCADTA | Colombia/Germany | D |
| Scandinavian Skies AS[6] | Norway | C |
| Sea To Sky Air[7] | Canada | C |
| Seabird Airlines | Turkey | D |
| Seaborne Airlines | United States | C |
| Seair Pacific | Australia | C |
| Seair Seaplanes | Canada | C |
| Seawings | United Arab Emirates | C |
| Seawings Venice | Italy | C |
| Serviços Aéreos Cruzeiro do Sul | Brazil | D |
| Shoreline Aviation | United States | D |
| Simplifly | Sri Lanka | C |
| Società Aerea Mediterranea | Italy | D |
| Società Anonima Navigazione Aerea | Italy | D |
| Spice Shuttle | India | C |
| Star Air Service | United States | D |
| Starratt Airways | Canada | D |
| Subic Seaplane | Philippines | C |
| Summit Air | Canada | C |
| Superior Airways | Canada | C |
| Svensk Lufttrafik | Sweden | D |
| Tailwind Air Service | United States | C |
| Taquan Air | United States | C |
| TEAL | New Zealand | D |
| Tofino Air | Canada | C |
| Trans Australia Airlines | Australia | D |
| Trans Maldivian Airways | Maldives | C |
| Trans-Provincial Airlines | Canada | D |
| Transair | Canada | D |
| Transportes Aéreos Bandeirantes | Brazil | D |
| Transwest Air | Canada | C |
| Tropic Ocean Airways | United States | C |
|
| Vancouver Island Air | Canada | C |
| VARIG | Brazil | D |
| Viação Aérea Santos Dumont | Brazil | D |
| Wardair | Canada | D |
| Waterfront Air | Hong Kong | C |
| West Norway Airlines | Norway | D |
| Westcoast Air | Canada | D |
| Western Canada Airways | Canada | D |
| Widerøe | Norway | F |
| Wien Alaska Airways | United States | D |
| Wilderness Seaplanes[8] | Canada | C |
| World Airways | United States | D |
